Principle of the Procedure

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human PGII, and the Human PGII standard plate wells that pre coated using protein related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP conjugated antibody specific to Human PGII . After TMB substrate solution is added, only those wells that contain Human PGII and HRP conjugated antibody will exhibit a change in color. The enzyme substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGII in the samples is then determined by comparing the OD of the samples to the standard curve.