Principle of the Procedure

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat TRACP 5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat TRACP 5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TRACP 5b, biotin conjugated antibody and enzyme conjugated Avidin will exhibit a change in color. The enzyme substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TRACP 5b in the samples is then determined by comparing the OD of the samples to the standard curve.