Quanticon™ Human AGR2(Anterior Gradient Protein 2) ELISA Kit
Principle of the Procedure
This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Human AGR2. Standards or samples containing Human AGR2 are added to the plate and reacted with capture antibody. A second anti-Human AGR2 antibody labeled biotin is then added and binds to Human AGR2 captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Human AGR2-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Human AGR2 in the samples is then calculated from the OD value by establishing a standard curve.
Product Name | Quanticon™ Human AGR2(Anterior Gradient Protein 2) ELISA Kit |
SKU | ABQ-ES96-AGR2hu |
Applications | ELISA |
Reactivity | Human |
Alternative Name | Anterior gradient protein 2 homolog, AG2 |
Standard | 10ng/mL. |
Sensitivity | 0.1 ng/mL. |
Detection Range | 0.16-10ng/mL. |
Sample Type | Serum, Plasma, Tissue homogenate and Other biological samples |
Sample Volume | 50 ÎĽL |
Assay Type | Competitive |
Assay Duration | 1.5H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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