Quanticon™ Human CNN1(Calponin 1, Basic, Smooth Muscle) ELISA Kit
Principle of the Procedure
This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Rat CMLC-1. Standards or samples containing Rat CMLC-1 are added to the plate and reacted with capture antibody. A second anti-Rat CMLC-1 antibody labeled biotin is then added and binds to Rat CMLC-1 captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Rat CMLC-1-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Rat CMLC-1 in the samples is then calculated from the OD value by establishing a standard curve.
Product Name  | Quanticon™ Rat CMLC-1(Cardiac Myosin Light Chain 1) ELISA Kit |
SKU | ABQ-ES96-CMLC1rt |
Applications | ELISA |
Reactivity | Rat |
Alternative Name | MYL3, CMH8, MLC1SB, MLC1V, VLC1, MLC-lV/sb, VLCl |
Standard | 10ng/mL. |
Sensitivity | 0.1 ng/mL. |
Detection Range | 0.16-10ng/mL. |
Sample Type | Serum, Plasma, Tissue homogenate Cell culture supernatant and Other biological samples |
Sample Volume | 100 ÎĽL |
Assay Type | Sandwich |
Assay Duration | 3.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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