Quanticon™ Human CSF2Rα(Colony Stimulating Factor 2 Receptor Alpha) ELISA Kit
Principle of the Procedure
This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Human CSF2Rα. Standards or samples containing Human CSF2Rα are added to the plate and reacted with capture antibody. A second anti-Human CSF2Rα antibody labeled biotin is then added and binds to Human CSF2Rα captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Human CSF2Rα-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Human CSF2Rα in the samples is then calculated from the OD value by establishing a standard curve.
Product Name
| Quanticon™ Human CSF2Rα(Colony Stimulating Factor 2 Receptor Alpha) ELISA Kit |
SKU | ABQ-ES96-CSF2RAhu |
Applications | ELISA |
Reactivity | Human |
Alternative Name | CSF2RA, CD116, CDw116, CSF2R, CSF2RAX, CSF2RAY, CSF2RX |
Standard | 2000pg/mL |
Sensitivity | 18.75 pg/mL |
Detection Range | 31.25-2000pg/mL |
Sample Type | Serum, Plasma, Tissue homogenate Cell culture supernatant and Other biological samples |
Sample Volume | 100 μL |
Assay Type | Sandwich |
Assay Duration | 3.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
Citations
